NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Cancer Immune Monitoring and Analysis Centers and Cancer Immunologic Data Commons Network (CIMAC-CIDC Network)

Description

This compendium contains SOPs contributed by the Cancer Immune Monitoring and Analysis Centers and Cancer Immunologic Data Commons Network (CIMAC-CIDC Network; https://cimac-network.org/), which was established as part of the Cancer Moonshot Initiative. SOPs are available at https://cimac-network.org/about/documents/ and https://cimac-network.org/assays/. In addition to the SOPs available in this compendium, data access/transfer guidelines and a human material transfer agreement are also available on the CIMAC-CIDC Network website (https://cimac-network.org/about/documents/).

Sources

  • CIMAC-CIDC Network

SOPs

Title Version (Release Date) Source
CIMAC Specimen Collection Umbrella CIMAC-CIDC Network
Multiplex-Immunofluorescence SOP (DFCI) CIMAC-CIDC Network
Multiplex ImmunFluorescence (mIF) SOP CIMAC-CIDC Network
Digital Image Analysis of Whole Slide Images CIMAC-CIDC Network
Multiplex Immunohistochemical Consecutive Staining on a Single Slide CIMAC-CIDC Network
CIMAC IHC Slide Scanner CIMAC-CIDC Network
Singleplex Chromogenic IHC CIMAC-CIDC Network
CD3 Immunohistochemistry CIMAC-CIDC Network
Scoring of Chromogenic IHC CIMAC-CIDC Network
CIMAC CyTOF Harmonized SOP/Approach CIMAC-CIDC Network
Analytic Validations - CyTOF Proteomics CIMAC-CIDC Network
RNAseq_MDACC CIMAC-CIDC Network
SOP RiboGreen - Detection of RNA on the BioTek Synergy Microplate Reader CIMAC-CIDC Network
RNA Extraction from Frozen Tissue via QIAamp RNeasy Mini Kit CIMAC-CIDC Network
Extraction of RNA from FFPE Tissue Sections CIMAC-CIDC Network
Nanostring SOP CIMAC-CIDC Network
Agilent SureSelect Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library Protocol CIMAC-CIDC Network
Extraction of DNA from FFPE Tissue Sections CIMAC-CIDC Network
Manual DNA Isolation from Frozen Tissue using 50 mL Tubes and the QIAamp DNA Mini Kit CIMAC-CIDC Network
SOP PicoGreen - Detection of dsDNA on the BioTek Synergy Microplate Reader CIMAC-CIDC Network
CIMAC Library Preparation Using TWIST Kit CIMAC-CIDC Network
Olink Assay using Proseek Multiplex Kit CIMAC-CIDC Network
Grand Serology ELISA CIMAC-CIDC Network
Download all SOP documents of selected versions [23]
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