NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Cancer Immune Monitoring and Analysis Centers and Cancer Immunologic Data Center Network (CIMAC-CIDC Network)

Description

This compendium contains assay SOPs and assay analytical validation/performance reports contributed by the Cancer Immune Monitoring and Analysis Centers and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), which was established as part of the Cancer Moonshot Initiative. In addition to the SOPs and analytical reports available in this compendium, the Network's human material transfer agreement (HMTA), covering specimen and data transfers, is also available on the CIMAC-CIDC Network website at the link above.

Sources

  • CIMAC-CIDC Network

SOPs

Title Version (Release Date) Source
CIMAC Specimen Collection Umbrella CIMAC-CIDC Network
Multiplex-Immunofluorescence SOP (DFCI) CIMAC-CIDC Network
Multiplex ImmunFluorescence (mIF) SOP (MD Anderson) CIMAC-CIDC Network
Mutiplex ImmunoFluorescence (mIF) Analytical Validation CIMAC-CIDC Network
Digital Image Analysis of Whole Slide Images CIMAC-CIDC Network
Multiplex Immunohistochemical Consecutive Staining on a Single Slide (MICSSS) CIMAC-CIDC Network
CIMAC IHC Slide Scanner CIMAC-CIDC Network
Singleplex Chromogenic IHC CIMAC-CIDC Network
CD3 Immunohistochemistry CIMAC-CIDC Network
Scoring of Chromogenic IHC CIMAC-CIDC Network
CIMAC CyTOF Harmonized SOP/Approach CIMAC-CIDC Network
Analytic Validation - CyTOF Proteomics CIMAC-CIDC Network
RNAseq_MDACC CIMAC-CIDC Network
SOP RiboGreen - Detection of RNA on the BioTek Synergy Microplate Reader CIMAC-CIDC Network
RNA Extraction from Frozen Tissue via QIAamp RNeasy Mini Kit CIMAC-CIDC Network
Extraction of RNA from FFPE Tissue Sections CIMAC-CIDC Network
Nanostring SOP CIMAC-CIDC Network
Nanostring Analytical Validation CIMAC-CIDC Network
Agilent SureSelect Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library Protocol CIMAC-CIDC Network
Extraction of DNA from FFPE Tissue Sections CIMAC-CIDC Network
Manual DNA Isolation from Frozen Tissue using 50 mL Tubes and the QIAamp DNA Mini Kit CIMAC-CIDC Network
SOP PicoGreen - Detection of dsDNA on the BioTek Synergy Microplate Reader CIMAC-CIDC Network
CIMAC Library Preparation Using TWIST Kit CIMAC-CIDC Network
Olink Assay using Proseek Multiplex Kit CIMAC-CIDC Network
Olink Analytical Validation CIMAC-CIDC Network
Grand Serology ELISA CIMAC-CIDC Network
ELISA / Grand Serology Analytical Validation Report CIMAC-CIDC Network
Analytic Validation: ATAC epigenome profiling CIMAC-CIDC Network
NanoString GeoMx Digital Spatial Profiling Analytical Validation of Immuno-oncology Protein Targets CIMAC-CIDC Network
Microbiome Analytical Validation CIMAC-CIDC Network
Microbiome: 16S V4 ribosomal RNA (rRNA) Sequencing Analytical Validation CIMAC-CIDC Network
Multiplex Immunofluorescence (mIF) Analytical Validation (vectra 9C) CIMAC-CIDC Network
MICSSS Multiplex IHC Analytical Validation (Mt Sinai) CIMAC-CIDC Network
Single Cell - ATAC- Sequencing Validation Report CIMAC-CIDC Network
Analytical Performance: scRNAseq (ISMMS) CIMAC-CIDC Network
Single Cell RNA Sequencing with TCR Enrichment Validation Report CIMAC-CIDC Network
Analytical Performance: Repertoire TCRseq using RNA CIMAC-CIDC Network
Analytical Performance: Visium Spatial RNA Sequencing CIMAC-CIDC Network
Whole Exome Sequencing Analytical Validation CIMAC-CIDC Network
Single-cell and bulk RNA-based TCR sequencing Analytical Validation CIMAC-CIDC Network
Download all SOP documents of selected versions [40]

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