NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Microbiome: 16S rRNA microbiome sequencing Analytical Validation (Mt Sinai)   Add to my SOP compendium

BRD ID: 3,107

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 57

Description

This assay analytical validation and performance report describes the methods used to validate the 16S microbiome sequencing assay for analysis of the metagenome from stool samples with the Illumina MiSeq platform. The document also presents data supporting the reported accuracy, precision, and analytical sensitivity and specificity. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at the Icahn School of Medicine at Mount Sinai. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

Analytical report_Microbiome_11042019_Mt Sinai_508_Final.pdf [Size: 899 KB]

Biospecimens
  • Fluid - Feces
  • Cell - Feces
Diagnoses
  • Normal
Topics
  • Biospecimen Processing
  • Analyte Extraction and Purification
  • Platform-specific Methodology

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