NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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MICSSS: Multiplex Immunohistochemical Consecutive Staining on a Single Slide (MICSSS) SOP (Mt Sinai)    Add to my SOP compendium

BRD ID: 3,284

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 137

Description

This document describes the procedures used by the Icahn School of Medicine at Mount Sinai, a Cancer Immune Monitoring and Analysis Center (CIMAC) site, for multiplex immunohistochemical chromogenic staining on a single slide (MICSSS). MICSSS uses consecutive immunohistochemical (IHC) staining, imaging, and destaining cycles to allow for the sequential staining of up to 10 markers on a single formalin-fixed, paraffin-embedded (FFPE) slide. The SOP is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

SOP_Automated MICSSS SOP_Mt_Sinai_110322_508_Final.pdf [Size: 1112 KB]
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Biospecimens
  • Tissue - Not specified
  • Tissue - General
Diagnoses
  • Neoplastic - Not specified
  • Not specified
Topics
  • Platform-specific Methodology

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