Single Cell RNA Multi-Omics Sequencing with TCR & BCR Repertoire Enrichment (BD Rhapsody system) Analytical Validation (Stanford)
BRD ID: 3,386
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 7
Description
This analytical validation and performance report describes the validation of the BD Rhapsody single cell RNA multi-omics sequencing (scRNAseq) assay using matched single cell T-cell receptor (TCR) and B-cell receptor (BCR) sequencing. The assay is designed for analysis of cryopreserved human or mouse T cell lines, B cell lines, peripheral blood mononuclear cells (PBMCs), and bone marrow cells. Assay accuracy was evaluated by analyzing the cartridge cell capture rate for each type of cell and by comparing the cell type distribution to the distribution obtained with flow cytometry. The document presents data supporting the precision, sensitivity, reference ranges, ruggedness and reproducibility of the assay. It is used by a the Cancer Immune Monitoring and Analysis Center (CIMAC) at the Stanford University, and is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm).
Documents for Download
508C_Rhapsody validation 050525_final w coverpage.pdf [Size: 812 KB]Biospecimens
- Fluid - Blood
- Cell - White Cells
- Cell - Bone Marrow
Diagnoses
- Neoplastic - Not specified
- General
- Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology