RNAseq: RNA Sequencing (IDT) Analytical Validation (MD Anderson
BRD ID: 3,444
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 61
Description
This analytical validation and performance report describes the methods used to validate the IDT xGen Library preparation kit with the xGen Exome Hyb Panel V2 exome capture RNA sequencing assay. Sensitivity and precision were assessed using Seraseq® reference materials. Specificity was evaluated using FFPE specimens from healthy individuals and tumor specimens. Accuracy was assessed by comparing results obtained for 54 FFPE specimens with those obtained using Nanostring’s nCounter® PanCancer Immune Profiling Panel. It is used by the Cancer Immune Monitoring and Analysis Center (CIMAC) at MD Anderson Cancer Center. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/research/networks/precision-medicine-oncology/cimac-cidc).
Documents for Download
MDACC RNA Sequencing Analytical Validation_IDT.pdf [Size: 2818 KB]Biospecimens
- Tissue - General
- Tissue - Not specified
- Cell - Blood
Diagnoses
- Not specified
- General
- Neoplastic - Carcinoma
- Neoplastic - Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology