RNAscope: Analytical Validation of RNAscope Assay for Immuno-oncology Biomarker Probes (MD Anderson)
BRD ID: 3,265
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 59
Description
This assay analytical validation and performance report describes the methods used to validate RNAscope probes (CTLA4, CD274 (PD-L1), IL10, IFNG, TNFRSF8 (CD30),
PPIB, and DapB) for RNAscope staining of formalin-fixed, paraffin-embedded (FFPE) tonsil and solid (lung cancer) specimens. The document describes the immune cell versus non-immune cell and tissue distribution of staining. The document presents data that supports the reported accuracy, precision, analytical sensitivity, and specificity of the probes. It is used by the Cancer Immune Monitoring and Analysis Center (CIMAC) at the MD Anderson Cancer Center. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network); https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
Analytical Report_MDACC_RNAscope_020124_508_Final.pdf [Size: 957 KB]Biospecimens
- Tissue - General
- Tissue - Lung
- Tissue - Tonsil
Diagnoses
- Neoplastic - Carcinoma
- Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology