EVP: Extracellular vesicle and particles small RNA sequencing Analytical Validation (Mt Sinai)
BRD ID: 3,144
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 54
Description
This assay analytical validation and performance report describes the methods used to validate the isolation of extracellular vesicles (EVs) and particles from plasma, serum, and urine and their analysis by RNA sequencing with size exclusion chromatography (SEC). The document also presents data supporting the reported accuracy, precision, and analytical sensitivity and specificity of the assay, as well as the minimal RNA input amount and optimal sample storage conditions. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at the Icahn School of Medicine at Mount Sinai. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
Analytical report_EVPsmallRNAseq_11222022_Mt Sinai_508_Final.pdf [Size: 1786 KB]Biospecimens
- Fluid - Blood
- Fluid - Serum
- Fluid - Plasma
- Fluid - Urine
Diagnoses
- Neoplastic - Carcinoma
- Neoplastic - Not specified
Topics
- Analyte Extraction and Purification
- Platform-specific Methodology
Associated SOP Compendiums
- Analyte Extraction and Purification
- Platform-specific Methodology