NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Singleplex Chromogenic IHC   Add to my SOP compendium

BRD ID: 2,690

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 1370

Description

This document details the procedure used by the MD Anderson Cancer Center, a Cancer Immune Monitoring and Analysis (CIMAC) site, for singleplex chromogenic immunohistochemistry of paraffin-embedded tissue sections for the following markers: CD3, CD4, CD45RO, CD57, CD68, CD8, Foxp3, Granzyme B, PD-1, PD-L1. It is a product of the CIMAC and Cancer Immunologic Data Commons (CIMAC-CIDC) Network (CIMAC-CIDC Network; https://cimac-network.org/), which was established as part of the Cancer Moonshot Initiative. SOPs are available at https://cimac-network.org/assays/.

Documents for Download

CIMAC Singleplex IHC_MDACC.pdf [Size: 969 KB]

Biospecimens
  • Tissue - Not specified
Diagnoses
  • Not specified
Topics
  • Platform-specific Methodology

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