Microbiome: 16S V4 Sequencing SOP (MD Anderson)
BRD ID: 3,266
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 78
Description
This document describes the procedure used by the MD Anderson Cancer Center at the University of Texas, a Cancer Immune Monitoring and Analysis Center (CIMAC) site, for isolating and sequencing 16S rRNA bacterial genomic DNA from fecal specimens. The SOP includes DNA isolation using a modified version of the QIAamp Fast DNA Stool Kit, frozen storage of DNA, DNA quantification by spectrometer, 16S V4 amplification, amplicon pooling, and amplicon purification by gel extraction. The SOP also contains a list of primer sequences. The SOP is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
SOP Microbiome 16S-V4-sequencing-SOP-111920 MDACC_508_Final.pdf [Size: 244 KB]Biospecimens
- Fluid - Feces
Diagnoses
- Not specified
- Neoplastic - Not specified
Topics
- Storage
- Analyte Extraction and Purification
- Platform-specific Methodology
Associated SOP Compendiums
- Storage
- Analyte Extraction and Purification
- Platform-specific Methodology