NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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RNAseq: RNA Sequencing Analytical Validation (MDACC)   Add to my SOP compendium

BRD ID: 3,164

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 255

Description

This document describes the procedures for and the analytical validation of a RNA sequencing (RNAseq) assay for analysis of frozen and formalin-fixed, paraffin-embedded (FFPE) lung tumor specimens and peripheral blood mononuclear cells (PBMCs). The document also presents data supporting the reported analytical performance, inter- and intra-assay reproducibility, analytical sensitivity, and concordance between FFPE and frozen specimens. It is used by the Cancer Immune Monitoring and Analysis Center (CIMAC) at MD Anderson Cancer Center (MDACC). It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/research/networks/precision-medicine-oncology/cimac-cidc).

Documents for Download

Analytical report_RNA seq_12022019_MDACC_508_Final.pdf [Size: 3493 KB]
Biospecimens
  • Tissue - Lung
  • Cell - White Cells
Diagnoses
  • Neoplastic - Carcinoma
Topics
  • Platform-specific Methodology

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