RNAseq: RNA Sequencing Analytical Validation (MDACC)
BRD ID: 3,164
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 65
Description
This document describes the procedures for and the analytical validation of a RNA sequencing (RNAseq) assay for analysis of frozen and formalin-fixed, paraffin-embedded (FFPE) lung tumor specimens and peripheral blood mononuclear cells (PBMCs). The document also presents data supporting the reported analytical performance, inter- and intra-assay reproducibility, analytical sensitivity, and concordance between FFPE and frozen specimens. It is used by the Cancer Immune Monitoring and Analysis Center (CIMAC) at MD Anderson Cancer Center (MDACC). It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
Analytical report_RNA seq_12022019_MDACC_508_Final.pdf [Size: 3493 KB]Biospecimens
- Tissue - Lung
- Cell - White Cells
Diagnoses
- Neoplastic - Carcinoma
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology