MICSSS: Multiplex IHC Analytical Validation (Mt Sinai)
BRD ID: 3,111
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 8
Description
This assay analytical validation and performance report describes the methods used to validate the multiplex immunohistochemical chromogenic staining on a single slide (MICSSS) assay using sections of formalin-fixed, paraffin-embedded (FFPE) tonsil specimens. The MICSSS assay uses iterative cycles of staining, analysis, and bleaching of up to 10 markers on a single FFPE slide. The document also presents data supporting (1) the reported accuracy, precision, analytical sensitivity and specificity, and reference intervals. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at Mount Sinai. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
Analytical report_Multiplex IHC MICSSS_02192019_Mt Sinai_508_Final.pdf [Size: 2248 KB]Biospecimens
- Tissue - Tonsil
- Tissue - Pancreas
- Tissue - Colorectal
- Tissue - Lymph Node
- Tissue - Placenta
Diagnoses
- Normal
- Neoplastic - Carcinoma
- Neoplastic - Not specified
Topics
- Storage
- Platform-specific Methodology
Associated SOP Compendiums
- Storage
- Platform-specific Methodology