scRNAseq: Single-Cell RNA sequencing Analytical Performance (Mt Sinai)
BRD ID: 3,115
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 8
Description
This assay analytical validation and performance report describes the methods used to validate a single cell RNA sequencing (scRNAseq) platform using a 10x Genomics Chromium workflow to analyze gene expression in peripheral blood mononuclear cells (PBMCs), bone marrow mononuclear cells, tumor, and tissue specimens. The document also presents data supporting the reported accuracy, precision, and analytical sensitivity. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at the Icahn School of Medicine at Mount Sinai. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
Analytical report_scRNAseq_03132023_Mt Sinai_Final.pdf [Size: 2157 KB]Biospecimens
- Cell - White Cells
- Cell - Bone Marrow
- Tissue - Not specified
Diagnoses
- Normal
- Neoplastic - Other
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology