NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Multiplex-Immunofluorescence SOP (DFCI)   Add to my SOP compendium

BRD ID: 2,685

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 1389


This document describes the procedures used at Dana-Farber Cancer Institute, a Cancer Immune Monitoring and Analysis Centers (CIMAC) site, for multiplex immunofluorescence analysis (SOX10, PD-L1, PD-1, CD8, and DAPI) of formalin-fixed paraffin-embedded (FFPE) slide specimens. It is a product of the CIMAC and Cancer Immunologic Data Commons (CIMAC-CIDC) Network (CIMAC-CIDC Network;, which was established as part of the Cancer Moonshot Initiative. SOPs are available at

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CIMAC multiplex Immunofluorescence_DFCI.pdf [Size: 132 KB]

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