NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Multiplex Immunofluorescence (mIF): SOP (DFCI)   Add to my SOP compendium

BRD ID: 2,685

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 1511

Description

This document describes the procedures used at Dana-Farber Cancer Institute, a Cancer Immune Monitoring and Analysis Center (CIMAC) site, for multiplex immunofluorescence analysis (SOX10, PD-L1, PD-1, CD8, and DAPI) of formalin-fixed paraffin-embedded (FFPE) slide specimens. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

CIMAC multiplex Immunofluorescence_DFCI.pdf [Size: 132 KB]
Biospecimens
  • Tissue - Not specified
Diagnoses
  • Not specified
  • Neoplastic - Not specified
Topics
  • Platform-specific Methodology

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