NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

CyTOF: CIMAC CyTOF Harmonized SOP/Approach   Add to my SOP compendium

BRD ID: 2,693

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 1298

Description

This document contains procedures for the cell surface staining of cryopreserved peripheral blood mononuclear cells (PBMCs) that were specifically adapted for multi-site comparisons. Please see the following publication for updated details on this SOP: Sahaf B, et al. Immune profiling mass cytometry assay harmonization: multicenter experience from CIMAC-CIDC. Clin Cancer Res. 27(18), 5062-5071 (2021)(https://pubmed.ncbi.nlm.nih.gov/34266889/). It is a product of the Cancer Immune Monitoring and Analysis Centers and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

CIMAC CyTOF_Harmonized.pdf [Size: 106 KB]

Biospecimens
  • Cell - White Cells
Diagnoses
  • Normal
  • Not specified
Topics
  • Platform-specific Methodology

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