snRNAseq: Single Nucleus RNA Sequencing Analytical Performance (DFCI)
BRD ID: 3,324
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 23
Description
This assay analytical validation and performance report describes the procedures used by the Cancer Immune Monitoring and Analysis Center (CIMAC) at Dana Farber Cancer Institute (DFCI) for the RNA sequencing of single nuclei from snap-frozen tissue. The analytical validation and performance report includes instructions for the snap-freezing and frozen storage of tissue specimens as well as the workflow for processing single nucleus RNA sequencing (snRNAseq) data. The document also presents data generated using myeloid cells, tumor cells, lymphocytes, pericytes, epithelial cells, and oligodendrocytes that support the reported accuracy, precision, analytical sensitivity and specificity of the assay. It is a product of the CIMAC and Cancer Immunologic Data Center (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
508C_Analytical_Performance_snRNAseq_DFCI_CIMAC_v2.0_clean_508 final.pdf [Size: 1088 KB]Biospecimens
- Cell - Lymph Node
- Cell - Muscle (Skeletal)
- Cell - Nerve
- Cell - General
- Tissue - Lymph Node
- Tissue - Muscle (Skeletal)
- Tissue - Nerve
- Tissue - General
Diagnoses
- Neoplastic - Not specified
- General
Topics
- Platform-specific Methodology
- Biospecimen Preservation
- Storage
Associated SOP Compendiums
- Platform-specific Methodology
- Biospecimen Preservation
- Storage