NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

NanoString nCounter: Analytical Validation (MD Anderson)   Add to my SOP compendium

BRD ID: 3,112

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 66

Description

This assay analytical validation and performance report describes the methods used to validate the nCounter Pan Cancer Panel in conjunction with the Nanostring's nCounter Analysis System using RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue specimens. The document also presents data supporting the reported analytical sensitivity using RNA input amounts of 0-625 ng, intra- and inter-assay reproducibility, inter-operator reproducibility, analytical specificity, and differentiation between normal and tumor specimens. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at MD Anderson Cancer Center. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

Analytical report_Nanostring_12032018_MDACC_508_Final rep.pdf [Size: 1939 KB]

Biospecimens
  • Tissue - Breast
  • Tissue - Tonsil
  • Tissue - Lung
Diagnoses
  • Normal
  • Neoplastic - Carcinoma
Topics
  • Platform-specific Methodology

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