NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

WES: CIMAC Library Preparation Using TWIST Kit SOP (MD Anderson)   Add to my SOP compendium

BRD ID: 2,714

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 1571

Description

This document describes the procedure used by the University of Texas MD Anderson Cancer Center, a Cancer Immune Monitoring and Analysis Center (CIMAC) site, for the preparation of samples for sequencing using the TWIST NGS target enrichment kit. The procedures are applicable for DNA isolated from fresh or formalin-fixed paraffin-embedded (FFPE) tissue, and blood specimens. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

Twist_CIMAC_Library_preparation_MDACC-CIMAC.pdf [Size: 291 KB]

Biospecimens
  • Tissue - Not specified
  • Cell - General
  • Fluid - General
Diagnoses
  • Not specified
  • Neoplastic - Not specified
Topics
  • Platform-specific Methodology

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