NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

CIMAC Library Preparation Using TWIST Kit   Add to my SOP compendium

BRD ID: 2,714

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 1376

Description

This document describes the procedure used by the University of Texas MD Anderson Cancer Center, a Cancer Immune Monitoring and Analysis (CIMAC) site, for the preparation of samples for sequencing using the TWIST NGS target enrichment kit. The procedures are applicable for DNA isolated from fresh or formalin-fixed paraffin-embedded (FFPE) tissue, and blood specimens. It is a product of the CIMAC and Cancer Immunologic Data Commons (CIMAC-CIDC) Network (CIMAC-CIDC Network; https://cimac-network.org/), which was established as part of the Cancer Moonshot Initiative. SOPs are available at https://cimac-network.org/assays/.

Documents for Download

Twist_CIMAC_Library_preparation_MDACC-CIMAC.pdf [Size: 291 KB]

Biospecimens
  • Tissue - Not specified
  • Cell - General
  • Fluid - General
Diagnoses
  • Not specified
Topics
  • Platform-specific Methodology

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