Microbiome: 16S V4 ribosomal RNA (rRNA) Sequencing Analytical Validation (MD Anderson)
BRD ID: 3,108
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 8
Description
This assay analytical validation and performance report describes the methods used to validate the 16S V4 ribosomal RNA (rRNA) sequencing assay for microbiome analysis of frozen stool specimens collected from healthy donors and patients who underwent stem cell transplants. The document also presents data supporting the reported accuracy, inter- and intra-assay reproducibility, and analytical accuracy. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at the MD Anderson Cancer Center. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
Analytical report_Microbiome_Sept 2020_MDACC_rev_508_Final.pdf [Size: 1616 KB]Biospecimens
- Fluid - Feces
- Cell - Feces
Diagnoses
- Normal
- Not specified
Topics
- Biospecimen Processing
- Analyte Extraction and Purification
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Analyte Extraction and Purification
- Platform-specific Methodology