NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Grand Serology ELISA: Analytical Validation (Mt Sinai)    Add to my SOP compendium

BRD ID: 3,106

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 118

Description

This assay analytical validation and performance report describes the methods used to validate a standard enzyme-linked immunosorbent assay (ELISA) and grand serology assay for multiple antigens to assess a patient's serological repertoire using plasma and serum from peripheral blood and bone marrow. The document also presents data supporting the reported accuracy, precision, analytical sensitivity and specificity, and cross-site harmonization. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at Mount Sinai. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

Analytical report_Grand serology ELISA_07252019_Mt Sinai_508_Final.pdf [Size: 2142 KB]

Biospecimens
  • Fluid - Blood
  • Fluid - Plasma
  • Fluid - Serum
  • Fluid - Bone Marrow
Diagnoses
  • General
  • Neoplastic - Melanoma
  • Neoplastic - Carcinoma
  • Normal
Topics
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Storage
  • Platform-specific Methodology

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