NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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scRNAseq / scATACseq: Nuclei Isolation for Single Cell ATAC and RNA Sequencing (Mt Sinai)   Add to my SOP compendium

BRD ID: 3,280

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 60

Description

This document describes the procedures used by the Icahn School of Medicine at Mount Sinai, a Cancer Immune Monitoring and Analysis Center (CIMAC) site, for nuclei isolation from cryopreserved peripheral blood mononuclear cells (PBMCs) or cell lines. The SOP is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

SOP_MtSinai_scRNAseq_SOP_Nuclei Isolation_042720_508_Final.pdf [Size: 184 KB]
Biospecimens
  • Cell - General
  • Cell - White Cells
Diagnoses
  • Not specified
  • Neoplastic - Not specified
Topics
  • Analyte Extraction and Purification

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