HLA Antibody-Based Immunoprecipitation Protocol for Immunopeptidomics (DFCI/Broad)
BRD ID: 3,465
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 2
Description
This document describes the procedures used at Dana-Farber Cancer Institute (DCFI), a Cancer Immune Monitoring and Analysis Center (CIMAC) site, for HLA-based immunoprecipitation and immunopeptidomic analysis by Liquid chromatography Mass Spectrometry (LC-MS/MS) to identify potential antigen targets of T-cell response. The protocol supports the following specimen input formats: 10-50 mg of frozen tumor, 1-10 million frozen cells, or OCT-embedded scrolls. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/research/networks/precision-medicine-oncology/cimac-cidc).
Documents for Download
Immunopedptidomics_DFCI_March 2026_508C.pdf [Size: 383 KB]Biospecimens
- Tissue - General
- Tissue - Not specified
- Fluid - Blood
- Cell - Blood
Diagnoses
- Neoplastic - Not specified
- General
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology