The expert-vetted NCI BEBP details blood collection and processing procedures specifically tailored for miRNA analysis. View the BEBP on BBRB’s website and in the BRD!
Announcement of a new NCI Biospecimen Evidence-Based Practices on blood collection and processing for miRNA analysis is available ahead of print in Clinical Chemistry Laboratory Medicine.
Stop by and learn more about:
NCI Biospecimen Evidence-Based Practices: Poster PA-06, Session 1 on Wednesday, May 3rd from 6:00-7:00 pm
Biospecimen and Data Resources from the Cancer Moonshot Biobank: Poster PG-07, Session 2 on Thursday, May 4 from 6:00 -7:00 pm
The top 5 most downloaded SOPs from the BRD's SOP Library were
SOPs have been downloaded from the Biospecimen Research Database’s SOP Library more than 1 million times!
Share SOPs used by your repository or biobank in NCI’s BRD today.
A new study from NCI's Biospecimen Pre-analytical Variables (BPV) Program is now available that reports effects on aCGH results in FFPE RCC specimens after a delay to fixation ≥3 h or a fixation time of 72 h.
New Contributing SOP Sources Include:
The top 5 most downloaded SOPs from the BRD are:
Cell Viability Testing by the NIEHS
Laboratory Shipping Protocols by the NIEHS
Whole Blood Washing Method by the Immune Tolerance Network
Vaginal Swab Collection by the GAPPS Repository
Click the links below to view SOPs added from each new Source organization.
University of Ottawa Heart Institute Biobank (UOHI Biobank)
Peripheral Neuropathy Research Registry (PNRR)
Jeffs 2019: Our newest Paper Suggestion reports a mass spec marker of thaw duration for plasma/serum. https://lnkd.in/d8NQcpRC
Click the links below to view the new SOPs that have recently been added to the BRD.
Columbia Center for Translational Immunology (CCTI) Biobank Core
European Association of Urology Research Foundation (EAU)
The May Editor's Choice Article for Journal of Histochemistry and Cytochemistry is "Impact of Preanalytical Factors on the Measurement of Tumor Tissue Biomarkers Using Immunohistochemistry" by Bagchi et al.
Effects of delay-to-fixation and time-in-fixative on IHC staining of cancer biomarkers
A compendium of SOPs related to the collection and testing for the novel 2019 coronavirus (SARS-CoV2, COVID-19) is available: https://brd.nci.nih.gov/brd/sop-compendium/show/1361.
The top 5 most downloaded SOPs from the BRD in 2020 were supplied by:
NIEHS: https://brd.nci.nih.gov/brd/sop/show/941
NIEHS: https://brd.nci.nih.gov/brd/sop/show/944
CDC: https://brd.nci.nih.gov/brd/sop/show/563
NIEHS: https://brd.nci.nih.gov/brd/sop/show/945
GAPPS Repository: https://brd.nci.nih.gov/brd/sop/show/890
Two new compendiums of SOPs related to snap-freezing tissue specimens are now available:
For SOPs that specify dry ice CLICK HERE.
For SOPs that specify liquid nitrogen CLICK HERE.
The NCI BEBP document for snap-freezing tissue can be found HERE.
CLICK HERE to view the ISBER 2020 electronic poster on the BRD.
View other's electronic posters on the ISBER 2020 main page HERE.
A new compendium of SOPs related to the collection and testing for the novel 2019 coronavirus (SARS-CoV2, COVID19) is now available: https://brd.nci.nih.gov/brd/sop-compendium/show/1361.
The BRD user survey will close this Friday, 7/31/20. Your responses will help us better tailor the BRD's content to the needs of the community. Consider taking the short 6 question survey by CLICKING HERE.
Please take a short survey on how you're using SOPs from the BRD by CLICKING HERE.
A new Expert-Vetted Biospecimen Evidence-Based Practices document on blood collection and processing for cfDNA analysis is now available.
Click here to view the BEBP document
Click here to view the accompanying publication
The top 5 most downloaded SOPs in the BRD in 2019 were supplied by:
NIEHS: https://brd.nci.nih.gov/brd/sop/show/941
NIEHS: https://brd.nci.nih.gov/brd/sop/show/944
NIEHS: https://brd.nci.nih.gov/brd/sop/show/945
CC GMI: https://brd.nci.nih.gov/brd/sop/show/823
GAPPS Repository: https://brd.nci.nih.gov/brd/sop/show/890
The top 5 most downloaded SOPs in the BRD in 2018 were supplied by:
NIEHS: https://brd.nci.nih.gov/brd/sop/show/941
NIEHS: https://brd.nci.nih.gov/brd/sop/show/944
GAPPS Repository: https://brd.nci.nih.gov/brd/sop/show/890
NIEHS: https://brd.nci.nih.gov/brd/sop/show/945
CC GMI: https://brd.nci.nih.gov/brd/sop/show/823
A new article from NCI's BPV Program is now available that reports deleterious effects of formalin-fixation and delays to fixation on RNA and miRNA-Seq profiles.
View the BRD Curation at https://brd.nci.nih.gov/brd/paper/sci-rep/2019/deleterious-effects-of-formalin-fixation-and-delays-to-fixation/129450
View the PubMed Abstract at https://www.ncbi.nlm.nih.gov/pubmed/31061401
Be sure to check out the presentation by NCI/BBRB's Helen Moore this Friday during the Biospecimen Science Session (4C), "The Evidence Base for Controlling and Mitigating Pre-analytical Variation".
At ISBER? Stop by and visit the BRD poster on quality assessment methods represented in the SOP Library (#PBR16).
Results from NCI's Biospecimen Pre-analytical Variables (BPV) Program on effects of a delay to fixation and time in fixative on DNA and RNA quality have been published. View the article at https://www.ncbi.nlm.nih.gov/pubmed/30785788.
View them at: https://brd.nci.nih.gov/brd/sop-compendium/show/1181.
SOPs from the Prostate Cancer Biorepository Network (PCBN) were added to the BRD. View them at: https://brd.nci.nih.gov/brd/sop-compendium/show/1141
A new Expert-Vetted Biospecimen Evidence-Based Practices document on nucleic acid extraction from FFPE tissue blocks is now available in the BRD's SOP library. Visit the publication announcing the release at https://www.ncbi.nlm.nih.gov/pubmed/29920119.
Don't miss BBRB's presentation on comparisons among SOPs from the BRD's SOP Library during this Wednesday's (5/23/18) morning session at the ISBER 2018 Annual Meeting.
Later, stop by and visit the ISBER Tool Bench at 3:30 pm (Wednesday, 5/23/18) to learn more about the BRD and how it may be of use to you.
Stop by and visit BBRB's poster (Abstract #4763) on the BRD and the Biospecimen Evidence-Based Practices document series at the AACR Annual Meeting 2018 on 4/17.
The newest edition of the ISBER Best Practices was released on January 31, 2018. View the document at http://www.isber.org/?page=BPR.
The top 5 most downloaded SOPs in the BRD in 2017 were supplied by:
NIEHS: https://brd.nci.nih.gov/brd/sop/show/944
NIEHS: https://brd.nci.nih.gov/brd/sop/show/941
GAPPS Repository: https://brd.nci.nih.gov/brd/sop/show/890
UCHC Tissue Repository: https://brd.nci.nih.gov/brd/sop/show/841
NIEHS: https://brd.nci.nih.gov/brd/sop/show/945
The review, which appears in a special issue of Seminars in Cancer Biology, summarizes reported effects of preanalytical variability on immunologically relevant biomarkers. Find it here.
Stop by and visit BBRB's poster on the NCI's Biospecimen Evidence-Based Practices (BEBP) document series at the Global Biobank Meeting in Stockholm, Sweden September 13th-15th.
Stop by and visit BBRB's poster on the BRD and the Biospecimen Evidence-Based Practices document series at the ISBER 2017 Annual Meeting on May 11th.
The top 5 most downloaded SOPs in the BRD in 2016 were supplied by
NIEHS: https://brd.nci.nih.gov/brd/sop/show/944, https://brd.nci.nih.gov/brd/sop/show/945
CC GMI: https://brd.nci.nih.gov/brd/sop/show/823
GAPPS Repository: https://brd.nci.nih.gov/brd/sop/show/890
UCHC Tissue Repository: https://brd.nci.nih.gov/brd/sop/show/841
New SOPs from the Consortium de Recherche en Oncologie Clinique du Québec (Q-CROC) were added to the BRD. View them at https://brd.nci.nih.gov/brd/sop-compendium/show/601.
Visit BBRB's poster on the BRD at the ISBER Regional Meeting in Bethesda, MD on Nov 7th and 8th. http://meetings.isber.org/regional2016/
SOPs from NIH's Epidemiology & Genomics Research Program were added to the BRD. View them at http://brd.nci.nih.gov/brd/sop-compendium/show/501
Look for a link to the BRD on the bottom of the PubMed abstract page, under "LinkOut-more resources".
The review appears in Cancer Research's OnlineFirst section, and both summarizes the challenges associated with molecular analysis of FFPE biospecimens as well as highlights analytical techniques and parameters that result in strong concordance with a fresh or frozen cohort.
Click here to read the article.
The ISBER Biospecimen Science Working Group has compiled hundreds of biospecimen science references from both the clinical and the environmental sectors: click here!
A new review summarizing the effects of FFPE processing factors on nucleic acid, protein, and morphological endpoints appears in the November 2014 issue of Arch Pathol Lab Med. The review was drafted using references housed in the BRD.
Click here to read the article.
Click here to read the accompanying editorial.
NCI BEBPs are literature-annotated procedural guidelines based upon entries contained within the BRD. The first BEBP in an anticipated series is now available at biospecimens.cancer.gov/resources/bebp.asp and addresses snap-freezing of post-surgical tissue biospecimens.
The BRD has been cited as a reference in
Browse by Analyte or Pre-analytical Factor, or increase search stringency by using the Advanced Search, which contains drop down menu options and a keyword search. Results of a Keyword Search can also be limited to studies that explored experimental comparisons by selecting the box on the Search Results page.