ATACseq: inTAC-Seq Protocol (Stanford)
BRD ID: 3,267
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 14
Description
This document describes the procedure used by the Cancer Immune Monitoring and Analysis Center (CIMAC) site at Stanford University for epigenomic profiling using a modification of the Assay for Transposase-Accessible Chromatin (ATAC) that uses sorted, fixed, and permeabilized cells, termed “intracellular fluorescent activated cell sorted transposase accessible chromatin sequencing” (inTAC). The SOP includes both the steps in the original AITAC protocol (lysis, transposition, DNA purification, amplification, and next-generation sequencing) and the additional steps for fixing and staining cells with the FoxP3 Fix/Perm Buffer Kit, sorting cells by FACS and reversing cross-linking. The SOP is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
SOP_ATACseq_inTAC_Stanford CIMAC-08.01.18_508_Final.pdf [Size: 539 KB]Biospecimens
- Cell - General
Diagnoses
- Not specified
- Neoplastic - Not specified
Topics
- Analyte Extraction and Purification
- Platform-specific Methodology
Associated SOP Compendiums
- Analyte Extraction and Purification
- Platform-specific Methodology