Microbiome: Microbiome DNA Isolation (Mt Sinai)
BRD ID: 3,276
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 48
Description
This document contains procedures used by the Icahn School of Medicine at Mount Sinai, a Cancer Immune Monitoring and Analysis Center (CIMAC) site, for the isolation of DNA from fecal specimens. The protocol outlines both mechanical lysis with zirconia/silica beads and chemical lysis with phenol/chloroform, followed by purification using a QIAquick 96 PCR Purification Kit. The isolated DNA is stored at -80°C. The SOP is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
SOP_Microbiome_DNA_Isolation_Mount Sinai_approved_508_Final.pdf [Size: 255 KB]Biospecimens
- Fluid - Feces
Diagnoses
- Neoplastic - Not specified
- Not specified
Topics
- Analyte Extraction and Purification
- Storage
Associated SOP Compendiums
- Analyte Extraction and Purification
- Storage