Multiplex Immunofluorescence (mIF): Panel Vectra 9C SOP (MD Anderson)
BRD ID: 3,273
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 19
Description
This document describes the procedure used by the MD Anderson Cancer Center at the University of Texas, a Cancer Immune Monitoring and Analysis Center (CIMAC) site, for automated multiplex immunofluorescence (mIF) staining of tissue sections with the Vectra 9C panel (CD34, CD3, CD45RO, FOXP3, PD1, PD-L1, and CD68) and a Bond RX instrument. The Bond Rx method allows for small reagent volumes (>150 µL). The SOP is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
SOP_MDACC_SOP_mIF_Panel9C_082922_508_Final.pdf [Size: 306 KB]Biospecimens
- Tissue - General
- Tissue - Not specified
Diagnoses
- Not specified
- Neoplastic - Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology