NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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scTCRseq: Standard Operating Procedure for Single Cell TCR Sequencing (sc-rhTCRseq1) (DFCI)   Add to my SOP compendium

BRD ID: 3,281

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 57

Description

This document describes the procedures used by the Cancer Immune Monitoring and Analysis Center (CIMAC) at Dana Farber Cancer Institute (DFCI) for RNA sequencing of single T-cells. The SOP includes instructions for frozen storage of single T-cells and cDNA libraries. It is a product of the CIMAC and Cancer Immunologic Data Center (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

SOP_TCRseq sc RNA_DFCI_12122019_Final_Signed_508_Final.pdf [Size: 446 KB]
Biospecimens
  • Cell - White Cells
Diagnoses
  • Not specified
  • Neoplastic - Not specified
Topics
  • Platform-specific Methodology
  • Storage

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