NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Spatial transcriptomics: NanoString GeoMx Digital Spatial Profiling Analytical Validation of Immuno-oncology Protein Targets (MD Anderson)   Add to my SOP compendium

BRD ID: 3,105

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 95

Description

This assay analytical validation and performance report describes the methods used to validate the Nanostring GeoMx Digital Spatial Profiling (DSP) Assay using formalin-fixed, paraffin-embedded (FFPE) normal tonsil, lung adenocarcinoma, specimens represented on a tissue microarray (normal and tumor, peripheral blood mononuclear cell (PBMC) specimens). The document also presents data supporting the reported accuracy, inter- and intra-assay reproducibility, and analytical sensitivity and specificity. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at MD Anderson Cancer Center. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

Analytical Report_GeoMx_082123_MDACC_508_Final rep.pdf [Size: 1785 KB]

Biospecimens
  • Tissue - Tonsil
  • Tissue - Lung
  • Tissue - Spleen
  • Tissue - Pancreas
  • Tissue - Placenta
  • Tissue - Brain
  • Tissue - Ovary
  • Tissue - Colorectal
  • Tissue - Skin
  • Tissue - Lymph Node
  • Tissue - Liver
  • Cell - White Cells
Diagnoses
  • Normal
  • Neoplastic - Carcinoma
  • Neoplastic - Lymphoma
Topics
  • Platform-specific Methodology

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