RNAscope: RNAscope In Situ Hybridization Assay SOP (MD Anderson)
BRD ID: 3,272
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 38
Description
This document describes the procedure used by the MD Anderson Cancer Center at the University of Texas, a Cancer Immune Monitoring and Analysis Center (CIMAC) site, for analysis of CTLA4, CD274 (PD-L1), IL10, IFNG, and TNFRSF8 (CD30) on formalin-fixed, paraffin-embedded sections using the in situ hybridization (ISH)-based RNAscope assay. The SOP is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.
Documents for Download
SOP_MDACC_RNAscope_121523_508_Final.pdf [Size: 312 KB]Biospecimens
- Tissue - General
- Tissue - Not specified
Diagnoses
- Not specified
- Neoplastic - Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology