NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

scATACseq: Single-Cell ATAC Sequencing Analytical Validation (MD Anderson)   Add to my SOP compendium

BRD ID: 3,114

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 82

Description

This assay analytical validation and performance report describes the methods used to validate a single cell Assay for Transposase-Accessible Chromatin (ATAC) sequencing for the analysis of cryopreserved melanoma tumor cells and tumor infiltrating lymphocytes (TIL) isolated from patients diagnosed with metastatic melanoma. The document also presents data supporting the reported accuracy, precision, and analytical sensitivity. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at MD Anderson Cancer Center. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

Analytical report_scATACseq_March 2021_MDACC_508_Final.pdf [Size: 9189 KB]

Biospecimens
  • Cell - Skin
  • Cell - White Cells
Diagnoses
  • Neoplastic - Melanoma
Topics
  • Platform-specific Methodology

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