CITE-Seq: Analytical Performance (DFCI)
BRD ID: 3,145
Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 277
Description
This assay analytical validation and performance report describes the methods used to validate the cellular indexing of transcriptomes and epitomes by sequencing (CITE-seq) assay for analysis of cryopreserved peripheral blood mononuclear cell (PBMC) samples. The document also presents data supporting the reported accuracy, precision, analytical sensitivity and specificity, range, and standardization of the assay, as well as quality control procedures. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at the Translational Immunogenomics Lab at the Dana-Farber Cancer Institute (DFCI). It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/research/networks/precision-medicine-oncology/cimac-cidc).
Documents for Download
Analytical report_CITE-seq_28Sep2021_DFCI_508_Final.pdf [Size: 2732 KB]Biospecimens
- Cell - White Cells
Diagnoses
- Neoplastic - Leukemia
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology