NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

scRNAseq: Single-Cell RNA Sequencing with TCR Enrichment Analytical Validation (MDACC)   Add to my SOP compendium

BRD ID: 3,116

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 10

Description

This assay analytical validation and performance report describes the methods used to validate a single cell RNA sequencing (scRNAseq) assay with matched single cell T-cell receptor (TCR) sequencing for the analysis of the immune repertoire and gene expression in the same cell; the assay was validated using cryopreserved melanoma tumor cells and tumor infiltrating lymphocytes (TIL) isolated from patients diagnosed with metastatic melanoma. The document also presents data supporting the reported accuracy, precision, and analytical sensitivity. It is used by a Cancer Immune Monitoring and Analysis Center (CIMAC) at MD Anderson Cancer Center. It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

Analytical report_scRNAseqTCR_071823_MDACC_508_Final.pdf [Size: 2443 KB]

Biospecimens
  • Cell - Skin
  • Cell - White Cells
Diagnoses
  • Neoplastic - Melanoma
Topics
  • Platform-specific Methodology

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