NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

scATACseq: Single-Cell ATAC-Sequencing Analytical Performance (DFCI)   Add to my SOP compendium

BRD ID: 3,166

Source: CIMAC-CIDC Network Tier: SOP Number of times downloaded: 107

Description

This assay analytical validation and performance report describes the methods used to validate a single-cell Assay for Transposase-Accessible Chromatin sequencing (scATAC-seq) for the analysis of cryopreserved peripheral blood mononuclear cells (PBMCs) isolated from a patient that relapsed with acute myeloid leukemia (AML). The document also presents data supporting the reported accuracy, precision, and analytical sensitivity and specificity. It is used by the Cancer Immune Monitoring and Analysis Center (CIMAC) at Dana-Farber Cancer Institute (DFCI). It is a product of the CIMAC and Cancer Immunologic Data Center Network (CIMAC-CIDC Network; https://dctd.cancer.gov/ResearchNetworks/cimacs_cidc_network.htm), a Cancer Moonshot Initiative.

Documents for Download

Analytical report_scATACseq_6Aug2021_DFCI_v8_508_Final.pdf [Size: 1655 KB]

Biospecimens
  • Cell - White Cells
Diagnoses
  • Neoplastic - Leukemia
Topics
  • Platform-specific Methodology

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