NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

DCTD Compendium

Description

The SOPs contained in this compendium are products of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). To download the most recent versions, please visit: http://dctd.cancer.gov/ResearchResources/ResearchResources-biomarkers.htm. To learn more about DCTD and their mission please visit their homepage at http://dctd.cancer.gov/.

Sources

  • DCTD

SOPs

Title Version (Release Date) Source
PBMC Protein Extraction for PAR Immunoassay DCTD
Biopsy Specimen Processing for PAR Immunoassay DCTD
Tumor Frozen Needle Biopsy Preparation for the gamma-H2AX IFA DCTD
PBMC Protein Extraction for TOP1 Immunoassay DCTD
HIF-1 alpha Immunoassay Tumor Frozen Needle Biopsy Specimen Collection and Handling DCTD
Tumor Frozen Needle Biopsy Specimen Collection and Handling DCTD
CellSave Tube: Human Blood Collection and Specimen Submission DCTD
PBMC Specimen Collection, Preparation, and Freezing for Protein Extraction DCTD
Biopsy Protein Extraction for the HIF-1 alpha Immunoassay DCTD
Tumor Biopsy Lysate Fractionation for the Apoptosis Multiplex Immunoassay Panels DCTD
Procedure for Use of Bio-Rad Bio-Plex Pro RBM Apoptosis Panel Kits 1 - 3 for the Analysis of Clinical Tumor Biopsy Extracts and Reporting of Data DCTD
Biopsy Specimen Processing for MET Immunoassay DCTD
Intact MET Immunoassay DCTD
Dual Phospho-Y1234/1235 MET Immunoassay DCTD
Phospho-Y1356 MET Immunoassay DCTD
Poly(ADP-ribose) (PAR) Immunoassay DCTD
Tumor Frozen Needle Biopsy Preparation for Pharmacodynamic Immunofluorescence Assays Utilizing Murine Testis and/or Jejunum Control Tissues DCTD
γH2AX, pNBS1 IFA Staining with β-Catenin Segmentation for Tumor Biopsy Slides DCTD
Tumor Biopsy Protein Extraction for Immunoassays DCTD
Procedure for use of Signaling Multiplex Panels 1-3 DCTD
Procedure for use of Signaling Multiplex Panels 4-5 (MEK/ERK) for the Analysis of Clinical Tumor Biopsy Extracts and Reporting of Data DCTD
Streck Tube: Human Blood Collection and Specimen Submission DCTD
γH2AX Immunofluorescence Assay Antibody Quantification and Laboratory Proficiency Testing DCTD
Immunofluorescence Assay for Circulating Tumor Cells Using the CellSearch System DCTD
PAR Immunoassay Quality Control, Data Analyses, and Reporting DCTD
Whole Slide Image Capture of Tumor Biopsy Slides for γH2AX, pNBS1 Immunofluorescence Assay with β-Catenin Segmentation DCTD
Image Extraction and Analysis of Tumor Biopsy Slides from γH2AX, pNBS1 IFA with β-Catenin Segmentation DCTD
EMT Panel IFA Staining for Tumor Biopsy Slides DCTD
Whole Slide Image Capture of Tumor Biopsy Slides for EMT Panel Immunofluoresence Assay DCTD
Image Extraction and Analysis of Tumor Biopsy Slides from EMT Panel IFA DCTD
Tumor Frozen Needle Biopsy Specimen Collection, Handling and Shipment to EET Biobank DCTD
Topoisomerase I Immunoassay DCTD
Biopsy Specimen Processing for TOP1 Immunoassay DCTD
TOP1 Immunoassay Quality Control, Data Analysis, and Reporting DCTD
HiF-1alpha Immunoassay DCTD
HIF-1 alpha Immunoassay Quality Control, Data Analyses, and Reporting DCTD
MET Immunoassay Quality Control, Data Analysis, and Reporting DCTD
Download all SOP documents of selected versions [37]

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