NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Tumor Frozen Needle Biopsy Preparation for the gamma-H2AX IFA   Add to my SOP compendium

BRD ID: 806

Source: DCTD Tier: SOP Number of times downloaded: 2107

Description

This document describes the method for fixing, embedding, and sectioning frozen needle biopsy specimens for use in immunofluorescence assays as well as assessing quality through hematoxylin and eosin (H&E) staining. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/biomarkers/DDR3/) for recent updates to the SOP.

Documents for Download

SOP340522_Biopsy_Section.pdf [Size: 386 KB]

Biospecimens
  • Tissue - General
Diagnoses
  • Neoplastic - Not specified
Topics
  • Biospecimen Acquisition
  • Biospecimen Preservation
  • Biospecimen Shipment
  • Platform-specific Methodology

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