NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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γH2AX Immunofluorescence Assay Antibody Quantification and Laboratory Proficiency Testing   Add to my SOP compendium

BRD ID: 3,030

Source: DCTD Tier: SOP Number of times downloaded: 140

Description

This document describes methods to qualify new batches of the γH2AX monoclonal antibody conjugated to Alexa Fluor 488 prior to use their use in immunofluorescence assays for circulating tumor cells (CTCs) with the Cell Search System (LHTP003.8.1). It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD; https://dctd.cancer.gov/). Please check the DCTD website (https://dctd.cancer.gov/drug-discovery-development/assays) for the current SOP version.

Documents for Download

LHTP003.8.1.1_CTC_gH2AX_QC_D.pdf [Size: 909 KB]
Biospecimens
  • Cell - Blood
Diagnoses
  • Neoplastic - Not specified
Topics
  • Platform-specific Methodology

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