NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
Advanced SearchBrowse by AnalyteBrowse by Pre-analytical FactorSearch SOPsSOP Compendiums

γH2AX Immunofluorescence Assay Antibody Quantification and Laboratory Proficiency Testing   Add to my SOP compendium

BRD ID: 3,030

Source: DCTD Tier: SOP Number of times downloaded: 71

Description

This document describes methods to qualify new batches of the γH2AX monoclonal antibody conjugated to Alexa Fluor 488 prior to use their use in immunofluorescence assays for circulating tumor cells (CTCs) with the Cell Search System (LHTP003.8.1). It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/ResearchResources-biomarkers.htm) for recent updates to the SOP.

Documents for Download

LHTP003.8.1.1_CTC_gH2AX_QC_D.pdf [Size: 909 KB]
Biospecimens
  • Cell - Blood
Diagnoses
  • Neoplastic - Not specified
Topics
  • Platform-specific Methodology

Associated SOP Compendiums

News and Announcements

  • New Articles on the GTEx Project are Now FREELY Available!
  • Just Published!
  • April 24, 2024: Biobanking for Precision Medicine Seminar
    • More...