NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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PBMC Protein Extraction for TOP1 Immunoassay   Add to my SOP compendium

BRD ID: 808

Source: DCTD Tier: SOP Number of times downloaded: 1079

Description

This document describes the method for preparing lysates of peripheral blood mononuclear cells (PBMC) for quantification of topoisomerase 1 (TOP1). It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/biomarkers/TopoisomeraseI.htm) for recent updates to the SOP.

Documents for Download

SOP340703_TOP1_PBMC_Extraction.pdf [Size: 242 KB]
Biospecimens
  • Fluid - Blood
  • Cell - White Cells
Diagnoses
  • General
Topics
  • Analyte Extraction and Purification

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