NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Immunofluorescence Assay for Circulating Tumor Cells Using the CellSearch System   Add to my SOP compendium

BRD ID: 3,031

Source: DCTD Tier: SOP Number of times downloaded: 266

Description

This document describes the immunomagnetic selection of circulating tumor cells (CTCs) and the detection of biomarkers by immunofluorescence assay using a qualified primary conjugated antibody and the CellSearch System CellTracks AutoPrep and Analyzer II. It is intended to be used in coordination with DCTD SOPs on specimen collection (LHTP00.08.03) and antibody qualification (LHTP003.8.1.1). It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD; https://dctd.cancer.gov/). Please check the DCTD website (https://dctd.cancer.gov/drug-discovery-development/assays) for the current SOP version.

Documents for Download

LHTP003.8.1_CTC_CellSearch_E.pdf [Size: 436 KB]
Biospecimens
  • Cell - Blood
Diagnoses
  • Neoplastic - Not specified
Topics
  • Biospecimen Processing
  • Platform-specific Methodology

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