NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Immunofluorescence Assay for Circulating Tumor Cells Using the CellSearch System   Add to my SOP compendium

BRD ID: 3,031

Source: DCTD Tier: SOP Number of times downloaded: 125

Description

This document describes the immunomagnetic selection of circulating tumor cells (CTCs) and the detection of biomarkers by immunofluorescence assay using a qualified primary conjugated antibody and the CellSearch System CellTracks AutoPrep and Analyzer II. It is intended to be used to in coordination with DCTD SOPs on specimen collection (LHTP00.08.03) and antibody qualification (LHTP003.8.1.1). It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/ResearchResources-biomarkers.htm) for recent updates to the SOP.

Documents for Download

LHTP003.8.1_CTC_CellSearch_E.pdf [Size: 436 KB]
Biospecimens
  • Cell - Blood
Diagnoses
  • Neoplastic - Not specified
Topics
  • Biospecimen Processing
  • Platform-specific Methodology

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