HIF-1alpha Immunoassay
BRD ID: 3,042
Source: DCTD Tier: SOP Number of times downloaded: 173
Description
This document describes procedures for quantifying hypoxia inducible factor-1 alpha (HIF-1α) in lysates from frozen tumor biopsies by enzyme-linked immunosorbent assay (ELISA). Guidance on the minimum required protein load per well is also provided. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD; https://dctd.cancer.gov/). Please check the DCTD website (https://dctd.cancer.gov/drug-discovery-development/assays) for the current SOP version.
Documents for Download
SOP340903_H1F1alpha_IA_C.pdf [Size: 424 KB]Biospecimens
- Tissue - Not specified
Diagnoses
- Neoplastic - Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology