NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Tumor Biopsy Lysate Fractionation for the Apoptosis Multiplex Immunoassay Panels   Add to my SOP compendium

BRD ID: 3,005

Source: DCTD Tier: SOP Number of times downloaded: 42

Description

This document describes the preparation of cytosolic and combined nuclear/mitochondrial cell fraction lysates from frozen needle biopsies for analyte quantification using multiplex immunoassays. It also includes steps to determine protein concentration by the bicinchoninic acid (BCA) assay. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/ResearchResources-biomarkers.htm) for recent updates to the SOP.

Documents for Download

SOP341401_ApoptosisPanel_Tumor_Extraction_C.pdf [Size: 699 KB]
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Biospecimens
  • Tissue - General
Diagnoses
  • Neoplastic - Not specified
  • General
Topics
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage
  • Platform-specific Methodology

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