NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Tumor Biopsy Lysate Fractionation for the Apoptosis Multiplex Immunoassay Panels   Add to my SOP compendium

BRD ID: 3,005

Source: DCTD Tier: SOP Number of times downloaded: 42


This document describes the preparation of cytosolic and combined nuclear/mitochondrial cell fraction lysates from frozen needle biopsies for analyte quantification using multiplex immunoassays. It also includes steps to determine protein concentration by the bicinchoninic acid (BCA) assay. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website ( for recent updates to the SOP.

Documents for Download

SOP341401_ApoptosisPanel_Tumor_Extraction_C.pdf [Size: 699 KB]
Download SOP documents of all versions [2]
  • Tissue - General
  • Neoplastic - Not specified
  • General
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage
  • Platform-specific Methodology

Associated SOP Compendiums

News and Announcements

  • Just Published!

  • April 24, 2024: Biobanking for Precision Medicine Seminar

  • Most Popular SOPs in March 2024

  • More...