NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Image Extraction and Analysis of Tumor Biopsy Slides from γH2AX, pNBS1 IFA with β-Catenin Segmentation   Add to my SOP compendium

BRD ID: 3,034

Source: DCTD Tier: SOP Number of times downloaded: 14

Description

This document describes the method for the extraction and analysis of whole slide images of formalin-fixed, paraffin-embedded (FFPE) tumor biopsy sections that were immunohistochemically stained for histone H2AX phosphorylated at serine 19 (γH2AX) and NBS1 phosphorylated at serine 343 (pNBS1) with β-Catenin tumor segmentation. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/ResearchResources-biomarkers.htm) for recent updates to the SOP.

Documents for Download

SOP340545_Image_Analyze_gH2AX_pNBS1_BCat_A.pdf [Size: 6211 KB]

Biospecimens
  • Tissue - Not specified
Diagnoses
  • Neoplastic - Not specified
Topics
  • Platform-specific Methodology

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