NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Biopsy Specimen Processing for MET Immunoassay   Add to my SOP compendium

BRD ID: 2,245

Source: DCTD Tier: SOP Number of times downloaded: 1543

Description

This document describes the preparation of lysates from frozen needle tumor biopsies to enable quantification of MET levels with an enzyme-linked immunosorbent assay (ELISA). It also includes steps to determine protein concentration by the bicinchoninic acid (BCA) assay. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/biomarkers/Intact_PhosphoMET.htm) for recent updates to the SOP.

Documents for Download

SOP341201_METIA_Tumor_Extraction.pdf [Size: 356 KB]
Biospecimens
  • Tissue - General
Diagnoses
  • General
  • Neoplastic - Not specified
Topics
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage
  • Platform-specific Methodology

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