Whole Slide Image Capture of Tumor Biopsy Slides for γH2AX, pNBS1 Immunofluorescence Assay with β-Catenin Segmentation
BRD ID: 3,033
Source: DCTD Tier: SOP Number of times downloaded: 96
Description
This document describes the method for capturing whole slide images of formalin-fixed, paraffin-embedded (FFPE) tumor biopsy sections following immunohistochemical staining for the quantification of histone H2AX phosphorylated at serine 19 (γH2AX) and NBS1 phosphorylated at serine 343 (pNBS1) with β-Catenin tumor segmentation. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/ResearchResources-biomarkers.htm) for recent updates to the SOP.
Documents for Download
SOP340544_Image_Capture_gH2AX_pNBS1_BCat_A.pdf [Size: 1000 KB]Biospecimens
- Tissue - Not specified
Diagnoses
- Neoplastic - Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology