NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Biopsy Protein Extraction for the HIF-1 alpha Immunoassay   Add to my SOP compendium

BRD ID: 813

Source: DCTD Tier: SOP Number of times downloaded: 1235

Description

This document describes the preparation of lysates from frozen fine needle biopsies and the determination of protein concentration by the bicinchoninic acid assay. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/biomarkers/hif1alpha.htm) for recent updates to the SOP.

Documents for Download

SOP340910_H1F1alpha_Biopsy_Extraction.pdf [Size: 381 KB]
Biospecimens
  • Tissue - General
Diagnoses
  • Neoplastic - Not specified
  • General
Topics
  • Analyte Extraction and Purification
  • Platform-specific Methodology

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