γH2AX, pNBS1 IFA Staining with β-Catenin Segmentation for Tumor Biopsy Slides
BRD ID: 2,251
Source: DCTD Tier: SOP Number of times downloaded: 761
Description
This document describes procedures used for immunohistochemical staining of formalin-fixed paraffin-embedded (FFPE) tissue biopsy sections with an automated processor for the detection and quantification of histone H2AX phosphorylated at serine 139 (γH2AX) and NBS1 phosphorylated at serine 343 (pNBS1) using β-Catenin staining. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/ResearchResources-biomarkers.htm) for recent updates to the SOP.
Documents for Download
SOP340543_Biopsy_BondRX_gH2AX_pNBS1_BCat.pdf [Size: 1818 KB]Biospecimens
- Tissue - General
Diagnoses
- General
- Neoplastic - Not specified
Topics
- Biospecimen Processing
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Platform-specific Methodology