NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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γH2AX, pNBS1 IFA Staining with β-Catenin Segmentation for Tumor Biopsy Slides   Add to my SOP compendium

BRD ID: 2,251

Source: DCTD Tier: SOP Number of times downloaded: 849

Description

This document describes procedures used for immunohistochemical staining of formalin-fixed paraffin-embedded (FFPE) tissue biopsy sections with an automated processor for the detection and quantification of histone H2AX phosphorylated at serine 139 (γH2AX) and NBS1 phosphorylated at serine 343 (pNBS1) using β-Catenin staining. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/ResearchResources-biomarkers.htm) for recent updates to the SOP.

Documents for Download

SOP340543_Biopsy_BondRX_gH2AX_pNBS1_BCat.pdf [Size: 1818 KB]
Biospecimens
  • Tissue - General
Diagnoses
  • General
  • Neoplastic - Not specified
Topics
  • Biospecimen Processing
  • Platform-specific Methodology

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