NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Biopsy Specimen Processing for PAR Immunoassay   Add to my SOP compendium

BRD ID: 804

Source: DCTD Tier: SOP Number of times downloaded: 1169

Description

This document describes the preparation of tissue and tumor lysates for ELISA quantification of poly ADP-ribose (PAR) and the determination of protein concentration by the bicinchoninic acid (BCA) assay. It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD; https://dctd.cancer.gov/). Please check the DCTD website (https://dctd.cancer.gov/drug-discovery-development/assays) for the current SOP version.

Documents for Download

SOP340520_Biopsy_Tissue.pdf [Size: 317 KB]
Biospecimens
  • Tissue - General
Diagnoses
  • Neoplastic - Not specified
  • General
Topics
  • Analyte Extraction and Purification
  • Platform-specific Methodology

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