NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

PBMC Protein Extraction for PAR Immunoassay   Add to my SOP compendium

BRD ID: 803

Source: DCTD Tier: SOP Number of times downloaded: 1205

Description

This document describes the preparation and storage of peripheral blood mononuclear cell (PBMC) lysates for ELISA quantification of poly ADP-ribose (PAR). It is a product of the National Cancer Institute's (NCI) Division of Cancer Treatment and Diagnosis (DCTD). Visit the DCTD website (https://dctd.cancer.gov/ResearchResources/biomarkers/PolyAdenosylRibose.htm) for recent updates to the SOP.

Documents for Download

SOP340506_PBMC_Extraction.pdf [Size: 219 KB]

Biospecimens
  • Cell - White Cells
  • Fluid - Blood
Diagnoses
  • General
Topics
  • Analyte Extraction and Purification

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