NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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The Cancer Genome Atlas (TCGA)

Description

The SOPs contained in this compendium are a product of The Cancer Genome Atlas Program (TCGA; https://cancergenome.nih.gov/) and were developed for use by the Biospecimen Core Resource (BCR; http://www.nationwidechildrens.org/biospecimen-core-resource-for-the-cancer-genome-atlas), a centralized laboratory and biorepository located within the Biopathology Center (BPC) at Nationwide Children's Hospital in Columbus, Ohio.

Sources

  • TCGA

SOPs

Title Version (Release Date) Source
H001 Standard Operating Procedure (SOP) for Hematoxylin and Eosin (H&E) Staining Coverslipping TCGA
H005 Standard Operating Procedure (SOP) for Automated Wright/Giemsa Staining for TCGA
H006 Standard Operating Procedure (SOP) for Sectioning Frozen Tissue Samples in Histology TCGA
H013 Standard Operating Procedure (SOP) for Paraffin Slides and Scrolls TCGA
H014 Standard Operating Procedure (SOP) for Scraping Slides for Extraction TCGA
L016 STANDARD OPERATING PROCEDURE (SOP) FOR PREPARING FROZEN TISSUE FOR MOLECULAR ANALYSIS TCGA
L017 STANDARD OPERATING PROCEDURE (SOP) FOR PREPARATION OF TISSUE SAMPLES FOR PROTEOME CHARACTERIZATION CENTERS TCGA
L020 Standard Operating Procedure (SOP) for Sectioning and Portioning Frozen Tissue Samples in Logistics TCGA
L024 Standard Operating Procedure (SOP) for Inbound Shipments TCGA
L025 Standard Operating Procedure (SOP) for Shipping an Outbound Package TCGA
M001 Standard Operating Procedure (SOP) for DNA/RNA Extraction with Allprep (DNA) and MirVana (Total RNA with Small RNA) TCGA
M002 Standard Operating Procedure (SOP) for RNA Nanoassay TCGA
M003 Standard Operating Procedure (SOP) for Gel Electrophoresis with the E-Gel System TCGA
M006 Standard Operating Procedure (SOP) for Normalization of Whole Genome Amplification Samples TCGA
M014 Standard Operating Procedure (SOP) for DNA Extraction from Blood with QIAMP MIDI Kit TCGA
M015 Standard Operating Procedure (SOP) for DNA Extraction With QIAamp MINI Kit TCGA
M017 STANDARD OPERATING PROCEDURE (SOP) FOR PICOGREEN DNA TCGA
M018 STANDARD OPERATING PROCEDURE (SOP) FOR SHIPPING ANALYTES TO DOWNSTREAM CENTERS TCGA
M019 STANDARD OPERATING PROCEDURE (SOP) FOR DNA/RNA Extraction with AllPrep (DNA) and mirVana (Total RNA with small RNA) 3 Column-Modified Melanoma Protocol TCGA
M020 STANDARD OPERATING PROCEDURE (SOP) FOR NINE MARKER MICROSATELLITE INSTABILITY (MSI) ANALYSIS BY POLYMERASE CHAIN REACTION TCGA
M022 STANDARD OPERATING PROCEDURE (SOP) FOR DNA/RNA EXTRACTION WITH ALLPREP (DNA) AND MIRVANA (TOTAL RNA WITH SMALL RNA) - MODIFIED LEUKEMIA PROTOCOL TCGA
M027 Standard Operating Procedure (SOP) for FFPE RNA/DNA Co-Isolation Using the Allprep FFPE Kit and the Highpure miRNA Kits TCGA
M028 Standard Operating Procedure (SOP) for Confirming Specimen Identity Using The Identifier Sequence-Specific Tandem Repeat (SSTR) Multiplex TCGA
Download all SOP documents of selected versions [23]

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