NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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M027 Standard Operating Procedure (SOP) for FFPE RNA/DNA Co-Isolation Using the Allprep FFPE Kit and the Highpure miRNA Kits   Add to my SOP compendium

BRD ID: 1,461

Source: TCGA Tier: SOP Number of times downloaded: 2528

Description

This document describes procedures for deparaffinization, decrosslinking, DNA extraction, and total RNA extraction and purification from unstained FFPE slides or tissue blocks. It is a product of The Cancer Genome Atlas (TCGA) Program and was developed for use by Biospecimen Core Resource (BCR), located within the Biopathology Center (BPC) at Nationwide Children's Hospital in Columbus, Ohio. More information on TCGA and the BCR is available at http://www.nationwidechildrens.org/biospecimen-core-resource-for-the-cancer-genome-atlas.

Documents for Download

Standard Operating Procedure (SOP) for FFPE RNA_DNA Co-Isolation Using the Allprep FFPE Kit and the Highpure miRNA Kits.pdf [Size: 545 KB]
Biospecimens
  • Tissue - General
Diagnoses
  • General
Topics
  • Analyte Extraction and Purification
  • Storage

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