NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

M022 STANDARD OPERATING PROCEDURE (SOP) FOR DNA/RNA EXTRACTION WITH ALLPREP (DNA) AND MIRVANA (TOTAL RNA WITH SMALL RNA) - MODIFIED LEUKEMIA PROTOCOL   Add to my SOP compendium

BRD ID: 1,460

Source: TCGA Tier: SOP Number of times downloaded: 1552

Description

This document describes procedures for DNA and RNA extraction from liquid acute myeloid leukemia (AML) tumors with the AllPrep DNA/RNA mini kit, and subsequent purification of total RNA with the mirVana miRNA Isolation kit. It is a product of The Cancer Genome Atlas (TCGA) Program and was developed for use by Biospecimen Core Resource (BCR), located within the Biopathology Center (BPC) at Nationwide Children's Hospital in Columbus, Ohio. More information on TCGA and the BCR is available at http://www.nationwidechildrens.org/biospecimen-core-resource-for-the-cancer-genome-atlas.

Documents for Download

STANDARD OPERATING PROCEDURE (SOP) FOR DNA_RNA EXTRACTION WITH ALLPREP (DNA) AND MIRVANA (TOTAL RNA WITH SMALL RNA) - MODIFIED LEUKEMIA PROTOCOL.pdf [Size: 215 KB]

Biospecimens
  • Fluid - Blood
  • Fluid - Bone Marrow
Diagnoses
  • Neoplastic - Leukemia
Topics
  • Analyte Extraction and Purification
  • Storage

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