M019 STANDARD OPERATING PROCEDURE (SOP) FOR DNA/RNA Extraction with AllPrep (DNA) and mirVana (Total RNA with small RNA) 3 Column-Modified Melanoma Protocol
BRD ID: 1,458
Source: TCGA Tier: SOP Number of times downloaded: 1797
Description
This document describes procedures for DNA and RNA extraction with the AllPrep DNA/RNA Mini kit from frozen melanoma and normal adjacent specimens, subsequent RNA purification with the mirVana miRNA Isolation kit, and melanin reduction of purified RNA with the cationic detergent cetyl- trimethylammonium bromide (CTAB).. It is a product of The Cancer Genome Atlas (TCGA) Program and was developed for use by Biospecimen Core Resource (BCR), located within the Biopathology Center (BPC) at Nationwide Children's Hospital in Columbus, Ohio. More information on TCGA and the BCR is available at http://www.nationwidechildrens.org/biospecimen-core-resource-for-the-cancer-genome-atlas.
Documents for Download
STANDARD OPERATING PROCEDURE (SOP) FOR DNA_RNA Extraction with AllPrep (DNA) and mirVana (Total RNA with small RNA) 3 Column-Modified Melanoma Protocol.pdf [Size: 372 KB]Biospecimens
- Tissue - Skin
Diagnoses
- Neoplastic - Melanoma
- Neoplastic - Normal Adjacent
Topics
- Analyte Extraction and Purification
- Storage
Associated SOP Compendiums
- Analyte Extraction and Purification
- Storage